XRCC3 Antibody

ProSci
Product Code: PSI-18-524
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-524-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HL-60 cells, using XRCC3 antibody (18-524) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat testis using XRCC3 antibody (18-524) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using XRCC3 antibody (18-524) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using XRCC3 antibody (18-524) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using XRCC3 antibody (18-524). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of HL-60 cells, using XRCC3 antibody (18-524) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat testis using XRCC3 antibody (18-524) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using XRCC3 antibody (18-524) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using XRCC3 antibody (18-524) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using XRCC3 antibody (18-524). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
DNA repair protein XRCC3, X-ray repair cross-complementing protein 3, CMM6
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 62-346 of human XRCC3 (NP_001093588.1).
NCBI Gene ID #:
7517
NCBI Official Name:
X-ray repair complementing defective repair in Chinese hamster cells 3
NCBI Official Symbol:
XRCC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O43542
User NOte:
Optimal dilutions for each application to be determined by the researcher.