NDRG1 Antibody

ProSci
Product Code: PSI-18-529
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-529-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NDRG1 antibody (18-529) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
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Immunofluorescence analysis of U2OS cells using NDRG1 antibody (18-529). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NDRG1 antibody (18-529) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Immunofluorescence analysis of U2OS cells using NDRG1 antibody (18-529). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
NDRG1, CAP43, CMT4D, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5, DRG-1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 175-394 of human NDRG1 (NP_001128714.1).
NCBI Gene ID #:
10397
NCBI Official Name:
N-myc downstream regulated 1
NCBI Official Symbol:
NDRG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
Q92597
User NOte:
Optimal dilutions for each application to be determined by the researcher.