PDGFR beta Antibody

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ProSci
Product Code: PSI-18-555
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-555-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PDGFR beta antibody (18-555) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
2 / 3
Immunofluorescence analysis of C6 cells using PDGFR beta antibody (18-555) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of NIH/3T3 cells using PDGFR beta antibody (18-555) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using PDGFR beta antibody (18-555) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
Immunofluorescence analysis of C6 cells using PDGFR beta antibody (18-555) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using PDGFR beta antibody (18-555) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
PDGFRB, CD140B, Beta pdfgr, Beta type PDGFR, BPDGFR, CD140b antigen, IBGC4, JTK12, PDGF-beta R, PDGF-R-beta, PDGFR, PDGFRbeta, PDGF receptor beta, PDGF-R, PDGFbetaR, PDGFR Beta, PDGFR-1, PDGFR-beta, BetaPDGFR, Pdgf beta-receptor, PDGFR1, PDGFRB/CEV14 fusion gene
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 887-1106 of human PDGFR beta (NP_002600.1).
NCBI Gene ID #:
5159
NCBI Official Name:
platelet-derived growth factor receptor, beta polypeptide
NCBI Official Symbol:
PDGFRB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 200kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Immunology, Signal Transduction
Swissprot #:
P09619
User NOte:
Optimal dilutions for each application to be determined by the researcher.