PPP2R2A Antibody

ProSci
Product Code: PSI-18-559
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-559-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PPP2R2A antibody (18-559) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat kidney using PPP2R2A antibody (18-559) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using PPP2R2A antibody (18-559) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using PPP2R2A antibody (18-559) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using PPP2R2A antibody (18-559) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using PPP2R2A antibody (18-559) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using PPP2R2A antibody (18-559) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PPP2R2A antibody (18-559) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform, PP2A subunit B isoform B55-alpha, PP2A subunit B isoform PR55-alpha, PP2A subunit B isoform R2-alpha, PP2A subunit B isoform alpha, PPP2R2A
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-447 of human PPP2R2A (NP_002708.1).
NCBI Gene ID #:
5520
NCBI Official Name:
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
NCBI Official Symbol:
PPP2R2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 52kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P63151
User NOte:
Optimal dilutions for each application to be determined by the researcher.