RPA2 Antibody

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ProSci
Product Code: PSI-18-562
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-562-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RPA2 antibody (18-562) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human kidney cancer using RPA2 Antibody (18-562) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using RPA2 antibody (18-562). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
4 / 4
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug RPA2 antibody (18-562). Western blot was performed from the immunoprecipitate using RPA2 antibody (18-562) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using RPA2 antibody (18-562) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human kidney cancer using RPA2 Antibody (18-562) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using RPA2 antibody (18-562). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug RPA2 antibody (18-562). Western blot was performed from the immunoprecipitate using RPA2 antibody (18-562) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
REPA2, RPA32, RPA34, Replication protein A 32 kDa subunit, Replication factor A protein 2, RP-A p32
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 80-270 of human RPA2 (NP_002937.1).
NCBI Gene ID #:
6118
NCBI Official Name:
replication protein A2, 32kDa
NCBI Official Symbol:
RPA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 32kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P15927
User NOte:
Optimal dilutions for each application to be determined by the researcher.