SELE Antibody

ProSci
Product Code: PSI-18-564
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-564-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SELE antibody (18-564) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human breast using SELE Antibody (18-564) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human uterus using SELE Antibody (18-564) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human uterine cancer using SELE Antibody (18-564) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using SELE antibody (18-564). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using SELE antibody (18-564) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human breast using SELE Antibody (18-564) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using SELE Antibody (18-564) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using SELE Antibody (18-564) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using SELE antibody (18-564). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
SELE, selectin E, CD62E, ELAM, ELAM1, ESEL, LECAM2, CD62 antigen-like family member E, E-selectin, ELAM-1, endothelial adhesion molecule 1, endothelial leukocyte adhesion molecule 1, leukocyte endothelial cell adhesion molecule 2, leukocyte-endothelial ce
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human SELE (NP_000441.2).
NCBI Gene ID #:
6401
NCBI Official Name:
selectin E
NCBI Official Symbol:
SELE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Stem Cell
Swissprot #:
P16581
User NOte:
Optimal dilutions for each application to be determined by the researcher.