UBE2I Antibody

ProSci
Product Code: PSI-18-566
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-566-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using UBE2I antibody (18-566) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 3
Immunohistochemistry of paraffin-embedded rat brain using UBE2I antibody (18-566) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse kidney using UBE2I antibody (18-566) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using UBE2I antibody (18-566) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using UBE2I antibody (18-566) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using UBE2I antibody (18-566) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
UBE2I, C358B7.1, P18, UBC9
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human UBE2I (NP_003336.1).
NCBI Gene ID #:
7329
NCBI Official Name:
ubiquitin-conjugating enzyme E2I
NCBI Official Symbol:
UBE2I
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 18kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Obesity
Swissprot #:
P63279
User NOte:
Optimal dilutions for each application to be determined by the researcher.