BRD4 Antibody

ProSci
Product Code: PSI-18-589
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-589-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using BRD4 antibody (18-589) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunofluorescence analysis of U-2 OS cells using BRD4 Polyclonal Antibody (18-589) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using BRD4 antibody (18-589) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of U-2 OS cells using BRD4 Polyclonal Antibody (18-589) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
BRD4, CAP, HUNKI, MCAP, HUNK1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human BRD4
NCBI Gene ID #:
23476
NCBI Official Name:
bromodomain containing 4
NCBI Official Symbol:
BRD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 200kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
O60885
User NOte:
Optimal dilutions for each application to be determined by the researcher.