EGLN2 Antibody

ProSci
Product Code: PSI-18-590
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-590-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EGLN2 antibody (18-590) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug EGLN2 antibody (18-590). Western blot was performed from the immunoprecipitate using EGLN2 antibody (18-590) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using EGLN2 antibody (18-590) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug EGLN2 antibody (18-590). Western blot was performed from the immunoprecipitate using EGLN2 antibody (18-590) at a dilition of 1:1000.

Further Information

Additional Names:
EGLN2, EIT6, PHD1, HPH-1, HPH-3, HIFPH1, HIF-PH1
Application Note:
WB: 1:500 - 1:2000

IP: 1:20 - 1:50
Background:
The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human EGLN2 (NP_444274.1).
NCBI Gene ID #:
112398
NCBI Official Name:
egl-9 family hypoxia-inducible factor 2
NCBI Official Symbol:
EGLN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 43kDa
Purification:
Affinity purification
Research Area:
Cancer
Swissprot #:
Q96KS0
User NOte:
Optimal dilutions for each application to be determined by the researcher.