HSPB2 Antibody

ProSci
Product Code: PSI-18-616
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-616-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HSPB2 antibody (18-616) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded mouse heart using HSPB2 antibody (18-616) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of U2OS cells using HSPB2 antibody (18-616). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HSPB2 antibody (18-616) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse heart using HSPB2 antibody (18-616) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using HSPB2 antibody (18-616). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HSP27, Hs.78846, LOH11CR1K, MKBP, heat shock protein beta-2, DMPK-binding protein, heat shock 27kDa protein 2, heat-shock protein beta-2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The protein is expressed preferentially in the heart and skeletal muscle. This protein regulates Myotonic Dystrophy Protein Kinase, which plays an important role in maintenance of muscle structure and function.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human HSPB2 (NP_001532.1).
NCBI Gene ID #:
3316
NCBI Official Name:
heat shock protein family B (small) member 2
NCBI Official Symbol:
HSPB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q16082
User NOte:
Optimal dilutions for each application to be determined by the researcher.