TriMethyl-Histone H3-K27 pAb

Product Code: PSI-18-626

Supplier: ProSci

Code	Size	Price

PSI-18-626-50uL

50uL

£505.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Shipping:

Blue Ice or RT

Storage:

Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (18-626) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

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Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (18-626).

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Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody (18-626) at dilution of 1:100 (40x lens).

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Immunohistochemistry of paraffin-embedded human breast using TriMethyl-Histone H3-K27 antibody (18-626) at dilution of 1:100 (40x lens).

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Immunohistochemistry of paraffin-embedded mouse kidney using TriMethyl-Histone H3-K27 antibody (18-626) at dilution of 1:100 (40x lens).

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Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K27 antibody (18-626). Blue: DAPI for nuclear staining.

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Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (Red)(18-626) and BrdU antibody (Green)(A1482). Blue: DAPI for nuclear staining.

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Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (18-626) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (18-626) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K27 antibody (18-625) at dilution of 1:100 (40x lens).

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Documents

Further Information

Additional Names:

H3F3A, H3t, H3.4, H3/g, H3FT

Application Note:

WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200

ChIP: 1:20 - 1:100

CHIP seq: 1:20 - 1:100

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Buffer:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration:

batch dependent

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3

Modifications:

Methylated

NCBI Gene ID #:

8290

NCBI Official Name:

histone cluster 3, H3

NCBI Official Symbol:

H3K27me3

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

Observed: 17kDa

Purification:

Affinity purification

Research Area:

Signal Transduction

Swissprot #:

Q16695

User NOte:

Optimal dilutions for each application to be determined by the researcher.