DiMethyl-Histone H3-K36 pAb

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ProSci
Product Code: PSI-18-628
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-628-50uL50uL£505.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K36 antibody (18-628).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K36 antibody (18-628).
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Immunohistochemistry of paraffin-embedded human breast using DiMethyl-Histone H3-K36 antibody (18-628) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K36 antibody (18-628) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H3-K36 antibody (18-628) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K36 antibody (18-628). Blue: DAPI for nuclear staining.
7 / 7
Chromatin immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K36 antibody (18-628) and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K36 antibody (18-628).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K36 antibody (18-628).
Immunohistochemistry of paraffin-embedded human breast using DiMethyl-Histone H3-K36 antibody (18-628) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K36 antibody (18-628) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H3-K36 antibody (18-628) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K36 antibody (18-628). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K36 antibody (18-628) and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Documents

Further Information

Additional Names:
H3F3A, H3t, H3.4, H3/g, H3FT
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200

ChIP: 1:20 - 1:100

CHIP seq: 1:20 - 1:100
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic methylated peptide corresponding to residues surrounding K36 of human histone H3
Modifications:
Methylated
NCBI Gene ID #:
8290
NCBI Official Name:
histone cluster 3, H3
NCBI Official Symbol:
H3K36me2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 18kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q16695
User NOte:
Optimal dilutions for each application to be determined by the researcher.