CDK1 Antibody

ProSci
Product Code: PSI-18-647
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-647-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CDK1 antibody (18-647) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human esophagus using CDK1 antibody (18-647) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using CDK1 antibody (18-647) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using CDK1 antibody (18-647) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human esophagus using CDK1 antibody (18-647) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CDK1 antibody (18-647) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
CDC2, CDC28A, CDK1, DKFZp686L20222, MGC111195, CDC2, P34CDC2
Application Note:
WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 208-258 of human CDK1 (NP_001777.1).
NCBI Gene ID #:
983
NCBI Official Name:
cyclin-dependent kinase 1
NCBI Official Symbol:
CDK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 34kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Neuroscience, Signal Transduction
Swissprot #:
P06493
User NOte:
Optimal dilutions for each application to be determined by the researcher.