EPB41 Antibody

ProSci
Product Code: PSI-18-664
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-664-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EPB41 antibody (18-664) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using EPB41 antibody (18-664) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using EPB41 antibody (18-664) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using EPB41 antibody (18-664). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using EPB41 antibody (18-664) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human colon carcinoma using EPB41 antibody (18-664) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EPB41 antibody (18-664) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EPB41 antibody (18-664). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
E41P, EPB4.1, band 4.1, P4.1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 210-500 of human EPB41 (NP_001159478.1).
NCBI Gene ID #:
2035
NCBI Official Name:
erythrocyte membrane protein band 4.1
NCBI Official Symbol:
EPB41
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 90-97kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P11171
User NOte:
Optimal dilutions for each application to be determined by the researcher.