NUP62 Antibody

ProSci
Product Code: PSI-18-670
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-670-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NUP62 antibody (18-670) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
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Immunohistochemistry of paraffin-embedded human lung cancer using NUP62 antibody (18-670) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using NUP62 antibody (18-670). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NUP62 antibody (18-670) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded human lung cancer using NUP62 antibody (18-670) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NUP62 antibody (18-670). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
NUP62, p62, MGC841, FLJ20822, DKFZp547L134, nucleoporin 62 kDa, nuclear pore glycoprotein p62, IBSN, SNDI
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 403-522 of human NUP62 (NP_036478.2).
NCBI Gene ID #:
23636
NCBI Official Name:
nucleoporin 62kDa
NCBI Official Symbol:
NUP62
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 62kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
P37198
User NOte:
Optimal dilutions for each application to be determined by the researcher.