SAT1 Antibody

ProSci
Product Code: PSI-18-675
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-675-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of THP-1 cells, using SAT1 antibody (18-675) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Western blot analysis of extracts of THP-1 cells, using SAT1 antibody (18-675) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Further Information

Additional Names:
DC21, KFSD, KFSDX, SAT, SSAT, SSAT-1, diamine acetyltransferase 1, diamine N-acetyltransferase 1, epididymis secretory sperm binding protein, polyamine N-acetyltransferase 1, putrescine acetyltransferase, spermidine/spermine N1-acetyltransferase alpha
Application Note:
WB: 1:500 - 1:2000
Background:
The protein encoded by this gene belongs to the acetyltransferase family, and is a rate-limiting enzyme in the catabolic pathway of polyamine metabolism. It catalyzes the acetylation of spermidine and spermine, and is involved in the regulation of the intracellular concentration of polyamines and their transport out of cells. Defects in this gene are associated with keratosis follicularis spinulosa decalvans (KFSD). Alternatively spliced transcripts have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-171 of human SAT1 (NP_002961.1).
NCBI Gene ID #:
6303
NCBI Official Name:
spermidine/spermine N1-acetyltransferase 1
NCBI Official Symbol:
SAT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P21673
User NOte:
Optimal dilutions for each application to be determined by the researcher.