EEF1D Antibody

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ProSci
Product Code: PSI-18-678
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-678-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EEF1D antibody (18-678) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat brain using EEF1D antibody (18-678) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using EEF1D antibody (18-678) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of U2OS cells using EEF1D antibody (18-678). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using EEF1D antibody (18-678) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using EEF1D antibody (18-678) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EEF1D antibody (18-678) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using EEF1D antibody (18-678). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
EF-1D, EF1D, FP1047, elongation factor 1-delta, EF-1-delta, antigen NY-CO-4, eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human EEF1D (NP_001123529.1).
NCBI Gene ID #:
1936
NCBI Official Name:
eukaryotic translation elongation factor 1 delta
NCBI Official Symbol:
EEF1D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P29692
User NOte:
Optimal dilutions for each application to be determined by the researcher.