PSMA4 Antibody

ProSci
Product Code: PSI-18-680
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-680-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PSMA4 antibody (18-680) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunofluorescence analysis of U2OS cells using PSMA4 antibody (18-680). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PSMA4 antibody (18-680) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using PSMA4 antibody (18-680). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
PSMA4, PSC9, proteasome subun, proteasome component C9, proteasome alpha 4 subunit, proteasome (prosome, macropain) subunit, alpha type, 4, multicatalytic endopeptidase complex subunit C9, MGC24813, MGC12467, MGC111191, macropain subunit C9, HsT17706, HC9
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PSMA4 (NP_001096137.1).
NCBI Gene ID #:
5685
NCBI Official Name:
proteasome (prosome, macropain) subunit, alpha type, 4
NCBI Official Symbol:
PSMA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P25789
User NOte:
Optimal dilutions for each application to be determined by the researcher.