NEDD9 Antibody

ProSci
Product Code: PSI-18-685
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-685-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of HeLa cells, using NEDD9 antibody (18-685) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 3
Immunohistochemistry of paraffin-embedded rat kidney using NEDD9 antibody (18-685) at dilution of 1:200 (40x lens).
3 / 3
Immunofluorescence analysis of U2OS cells using NEDD9 antibody (18-685). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of HeLa cells, using NEDD9 antibody (18-685) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using NEDD9 antibody (18-685) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using NEDD9 antibody (18-685). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
NEDD9, CAS-L, CASL, HEF1, dJ49G10.2, dJ761I2.1, CAS2, CASS2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-174 of human NEDD9 (NP_892011.2).
NCBI Gene ID #:
4739
NCBI Official Name:
neural precursor cell expressed, developmentally down-regulated 9
NCBI Official Symbol:
NEDD9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 115kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Signal Transduction
Swissprot #:
Q14511
User NOte:
Optimal dilutions for each application to be determined by the researcher.