DKK3 Antibody

ProSci
Product Code: PSI-18-691
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-691-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse brain, using DKK3 antibody (18-691) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat heart using DKK3 antibody (18-691) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using DKK3 antibody (18-691) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH-3T3 cells using DKK3 Polyclonal Antibody (18-691) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse brain, using DKK3 antibody (18-691) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using DKK3 antibody (18-691) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DKK3 antibody (18-691) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using DKK3 Polyclonal Antibody (18-691) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
DKK3, dickkopf homolog 3 (Xenopus laevis), REIC, DKK-3, RIG-like 5-6, RIG-like 7-1, dickkopf 3, Dickkopf gene 3, dickkopf (Xenopus laevis) homolog 3, dickkopf homolog 3, UNQ258/PRO295
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-350 of human DKK3 (NP_037385.2).
NCBI Gene ID #:
27122
NCBI Official Name:
dickkopf WNT signaling pathway inhibitor 3
NCBI Official Symbol:
DKK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
Q9UBP4
User NOte:
Optimal dilutions for each application to be determined by the researcher.