TMPO Antibody, KO Validated

ProSci
Product Code: PSI-18-696
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-696-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TMPO antibody (18-696) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Western blot analysis of extracts from normal (control) and TMPO knockout (KO) 293T cells, using TMPO antibody (18-696) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded rat brain using TMPO Antibody (18-696) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human tonsil using TMPO Antibody (18-696) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using TMPO Antibody (18-696) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using TMPO antibody (18-696).

Western blot analysis of extracts of various cell lines, using TMPO antibody (18-696) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts from normal (control) and TMPO knockout (KO) 293T cells, using TMPO antibody (18-696) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using TMPO Antibody (18-696) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using TMPO Antibody (18-696) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TMPO Antibody (18-696) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TMPO antibody (18-696).

Further Information

Additional Names:
Thymopoietin Antibody: TP, LAP2, CMD1T, LEMD4, PRO0868, Lamina-associated polypeptide 2, isoform alpha, Thymopoietin isoform alpha, TP alpha
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. Mutations in this gene are associated with dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human TMPO (NP_001027454.1).
NCBI Gene ID #:
7112
NCBI Official Name:
thymopoietin
NCBI Official Symbol:
TMPO
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 51kDa/80kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P42166/P42167
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.