TGFBI Antibody

ProSci
Product Code: PSI-18-712
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-712-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TGFBI antibody (18-712) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human lung cancer using TGFBI antibody (18-712) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using TGFBI antibody (18-712) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using TGFBI antibody (18-712) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using TGFBI antibody (18-712) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human lung cancer using TGFBI antibody (18-712) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TGFBI antibody (18-712) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TGFBI antibody (18-712) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
TGFBI, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 481-683 of human TGFBI (NP_000349.1).
NCBI Gene ID #:
7045
NCBI Official Name:
transforming growth factor, beta-induced, 68kDa
NCBI Official Symbol:
TGFBI
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 75kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Neuroscience, Signal Transduction
Swissprot #:
Q15582
User NOte:
Optimal dilutions for each application to be determined by the researcher.