WNK1 Antibody

ProSci
Product Code: PSI-18-716
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-716-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using WNK1 antibody (18-716) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of HeLa cells using WNK1 antibody (18-716). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using WNK1 antibody (18-716) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using WNK1 antibody (18-716). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
WNK1, Erythrocyte 65 kDa protein, HSAN2, KIAA0344, Kinase deficient protein, HWNK1, PHA2C, PRKWNK1, PSK, HSN2, KDP
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human WNK1 (NP_055638.2).
NCBI Gene ID #:
65125
NCBI Official Name:
WNK lysine deficient protein kinase 1
NCBI Official Symbol:
WNK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 300kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9H4A3
User NOte:
Optimal dilutions for each application to be determined by the researcher.