MAP2 Antibody

ProSci
Product Code: PSI-18-718
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-718-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MAP2 antibody (18-718) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5min.
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Immunohistochemistry of paraffin-embedded rat heart using MAP2 Antibody (18-718) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human kidney cancer using MAP2 Antibody (18-718) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using MAP2 Antibody (18-718) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using MAP2 antibody (18-718) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5min.
Immunohistochemistry of paraffin-embedded rat heart using MAP2 Antibody (18-718) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using MAP2 Antibody (18-718) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MAP2 Antibody (18-718) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
MAP2, MAP2C, MAP-2, MAP2A, MAP2B
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 260-559 of human MAP2 (NP_001034627.1).
NCBI Gene ID #:
4133
NCBI Official Name:
microtubule-associated protein 2
NCBI Official Symbol:
Map2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
P11137
User NOte:
Optimal dilutions for each application to be determined by the researcher.