KRT7 Antibody

ProSci
Product Code: PSI-18-719
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-719-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HeLa cells, using KRT7 antibody (18-719).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human placenta using KRT7 antibody (18-719) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of human lung cancer cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of rat bronchus cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of mouse bronchus cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of human lung cancer cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of rat bronchus cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of HeLa cells, using KRT7 antibody (18-719).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human placenta using KRT7 antibody (18-719) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of human lung cancer cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat bronchus cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse bronchus cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human lung cancer cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat bronchus cells using KRT7 antibody (18-719) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, KRT7, SCL
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 230-469 of human KRT7 (NP_005547.3).
NCBI Gene ID #:
3855
NCBI Official Name:
keratin 7
NCBI Official Symbol:
KRT7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 51kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P08729
User NOte:
Optimal dilutions for each application to be determined by the researcher.