SDHA Antibody

ProSci
Product Code: PSI-18-730
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-730-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SDHA antibody (18-730) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
2 / 5
Immunohistochemistry of paraffin-embedded rat heart using SDHA antibody (18-730) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human liver cancer using SDHA antibody (18-730) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded human esophageal cancer using SDHA antibody (18-730) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using SDHA antibody (18-730). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using SDHA antibody (18-730) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat heart using SDHA antibody (18-730) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SDHA antibody (18-730) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using SDHA antibody (18-730) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SDHA antibody (18-730). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial, Flavoprotein subunit of complex II, Fp, SDHA, SDH2, SDHF
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 385-664 of human SDHA (NP_004159.2).
NCBI Gene ID #:
6389
NCBI Official Name:
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
NCBI Official Symbol:
SDHA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P31040
User NOte:
Optimal dilutions for each application to be determined by the researcher.