Occludin Antibody

ProSci
Product Code: PSI-18-733
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-733-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Occludin antibody (18-733) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat pancreas using Occludin antibody (18-733) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using Occludin antibody (18-733) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using Occludin antibody (18-733) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using Occludin antibody (18-733) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of PC12 cells using Occludin antibody (18-733) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using Occludin antibody (18-733) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat pancreas using Occludin antibody (18-733) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Occludin antibody (18-733) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using Occludin antibody (18-733) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Occludin antibody (18-733) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using Occludin antibody (18-733) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Occludin, OCLN
Application Note:
WB: 1:1000 - 1:2000
Background:
This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 263-522 of human Occludin (NP_002529.1).
NCBI Gene ID #:
100506658
NCBI Official Name:
Occludin
NCBI Official Symbol:
OCLN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 59kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Signal Transduction
Swissprot #:
Q16625
User NOte:
Optimal dilutions for each application to be determined by the researcher.