COX5B Antibody

ProSci
Product Code: PSI-18-748
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-748-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using COX5B antibody (18-748) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded mouse heart using COX5B antibody (18-748) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver damage using COX5B antibody (18-748) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using COX5B antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using COX5B antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using COX5B antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug COX5B antibody (18-748). Western blot was performed from the immunoprecipitate using COX5B antibody (18-748) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using COX5B antibody (18-748) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse heart using COX5B antibody (18-748) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using COX5B antibody (18-748) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using COX5B antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using COX5B antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using COX5B antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HepG2 cells, using 3 ug COX5B antibody (18-748). Western blot was performed from the immunoprecipitate using COX5B antibody (18-748) at a dilition of 1:1000.

Further Information

Additional Names:
Cytochrome c oxidase subunit 5B, mitochondrial, Cytochrome c oxidase polypeptide Vb, COX5B
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 of human COX5B (NP_001853.2).
NCBI Gene ID #:
1329
NCBI Official Name:
Cytochrome c oxidase subunit 5B, mitochondrial
NCBI Official Symbol:
COX5B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
P10606
User NOte:
Optimal dilutions for each application to be determined by the researcher.