KRT5 Antibody

ProSci
Product Code: PSI-18-754
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-754-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using KRT5 antibody (18-754) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 4
Immunohistochemistry of paraffin-embedded human kidney using KRT5 Antibody (18-754) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human uterine cancer using KRT5 Antibody (18-754) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of HeLa cells using KRT5 antibody (18-754).

Western blot analysis of extracts of various cell lines, using KRT5 antibody (18-754) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human kidney using KRT5 Antibody (18-754) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using KRT5 Antibody (18-754) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using KRT5 antibody (18-754).

Further Information

Additional Names:
KRT5, 58 kd cytokeratin, 58 kDa cytokeratin, CK5, CK-5, Cytokeratin-5, DDD, EBS2, Keratin 5, KRT5A, Type-II keratin Kb5, Cytokeratin 5, K5, Keratin-5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human KRT5
NCBI Gene ID #:
3852
NCBI Official Name:
keratin 5
NCBI Official Symbol:
KRT5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P13647
User NOte:
Optimal dilutions for each application to be determined by the researcher.