FGF12 Antibody

ProSci
Product Code: PSI-18-759
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-759-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using FGF12 antibody (18-759) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat testis using FGF12 antibody (18-759) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using FGF12 antibody (18-759) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using FGF12 antibody (18-759) at dilution of 1:150 (40x lens).

Western blot analysis of extracts of various cell lines, using FGF12 antibody (18-759) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using FGF12 antibody (18-759) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using FGF12 antibody (18-759) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using FGF12 antibody (18-759) at dilution of 1:150 (40x lens).

Further Information

Additional Names:
EIEE47B, FHF1, FGF12, fibroblast growth factor 12, fibroblast growth factor 12B, fibroblast growth factor FGF-12b, fibroblast growth factor homologous factor 1, myocyte-activating factor
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human FGF12 (NP_004104.3).
NCBI Gene ID #:
2257
NCBI Official Name:
fibroblast growth factor 12
NCBI Official Symbol:
FGF12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 27kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology, Signal Transduction
Swissprot #:
P61328
User NOte:
Optimal dilutions for each application to be determined by the researcher.