CDK10 Antibody

ProSci
Product Code: PSI-18-768
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-768-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CDK10 antibody (18-768) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse brain using CDK10 antibody (18-768) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using CDK10 antibody (18-768) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CDK10 antibody (18-768) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using CDK10 antibody (18-768) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CDK10 antibody (18-768) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CDK10, cyclin-dependent kinase 10, PISSLRE, CDC2-related protein kinase, cell division protein kinase 10, cyclin-dependent kinase (CDC2-like) 10, cyclin-dependent kinase related protein, serine/threonine protein kinase PISSLRE
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to the CDK subfamily of the Ser/Thr protein kinase family. The CDK subfamily members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and are known to be essential for cell cycle progression. This kinase has been shown to play a role in cellular proliferation and its function is limited to cell cycle G2-M phase. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human CDK10 (NP_001092003.2).
NCBI Gene ID #:
8558
NCBI Official Name:
cyclin-dependent kinase 10
NCBI Official Symbol:
CDK10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 46kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q15131
User NOte:
Optimal dilutions for each application to be determined by the researcher.