DBH Antibody

ProSci
Product Code: PSI-18-778
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-778-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse brain, using DBH antibody (18-778) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human stomach using DBH antibody (18-778) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using DBH antibody (18-778) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of mouse brain, using DBH antibody (18-778) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using DBH antibody (18-778) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using DBH antibody (18-778) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
DBH, dopamine beta-hydroxylase (dopamine beta-monooxygenase), dopamine beta-hydroxylase, DBM
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100
Background:
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human DBH (NP_000778.3).
NCBI Gene ID #:
1621
NCBI Official Name:
dopamine beta-hydroxylase (dopamine beta-monooxygenase)
NCBI Official Symbol:
DBH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 79kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience
Swissprot #:
P09172
User NOte:
Optimal dilutions for each application to be determined by the researcher.