E2F6 Antibody

ProSci
Product Code: PSI-18-784
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-784-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of MCF-7 cells, using E2F6 antibody (18-784) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human esophageal cancer using E2F6 antibody (18-784) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using E2F6 antibody (18-784) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using E2F6 antibody (18-784). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 3ug E2F6 antibody (18-784). Western blot was performed from the immunoprecipitate using E2F6 antibody (18-784) at a dilition of 1:500.

Western blot analysis of extracts of MCF-7 cells, using E2F6 antibody (18-784) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human esophageal cancer using E2F6 antibody (18-784) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using E2F6 antibody (18-784) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using E2F6 antibody (18-784). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 3ug E2F6 antibody (18-784). Western blot was performed from the immunoprecipitate using E2F6 antibody (18-784) at a dilition of 1:500.

Further Information

Additional Names:
E2F6, E2F-6
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).
NCBI Gene ID #:
1876
NCBI Official Name:
E2F transcription factor 6
NCBI Official Symbol:
E2F6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Signal Transduction, Transcription
Swissprot #:
O75461
User NOte:
Optimal dilutions for each application to be determined by the researcher.