TRAP1 Antibody

ProSci
Product Code: PSI-18-800
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-800-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TRAP1 antibody (18-800) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 5
Immunohistochemistry of paraffin-embedded rat skeletal muscle using TRAP1 Antibody (18-800) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using TRAP1 Antibody (18-800) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using TRAP1 Antibody (18-800) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using TRAP1 antibody (18-800). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using TRAP1 antibody (18-800) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat skeletal muscle using TRAP1 Antibody (18-800) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TRAP1 Antibody (18-800) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TRAP1 Antibody (18-800) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using TRAP1 antibody (18-800). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
TRAP1, HSP75, HSP90L, HSP 75, TRAP-1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human TRAP1
NCBI Gene ID #:
10131
NCBI Official Name:
TNF receptor-associated protein 1
NCBI Official Symbol:
TRAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Chemokines & Cytokines, Immunology, Signal Transduction
Swissprot #:
Q12931
User NOte:
Optimal dilutions for each application to be determined by the researcher.