IGF2BP2 Antibody

ProSci
Product Code: PSI-18-801
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-801-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using IGF2BP2 antibody (18-801) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded mouse liver using IGF2BP2 antibody (18-801) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using IGF2BP2 antibody (18-801) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using IGF2BP2 antibody (18-801) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse liver using IGF2BP2 antibody (18-801) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using IGF2BP2 antibody (18-801) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
insulin-like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, VICKZ2, p62, IGF II mRNA binding protein 2, IGF-II mRNA-binding protein 2
Application Note:
WB: 1:200 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternative promoter usage and alternate splicing result in multiple variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human IGF2BP2 (NP_006539.3).
NCBI Gene ID #:
10644
NCBI Official Name:
insulin-like growth factor 2 mRNA binding protein 2
NCBI Official Symbol:
IGF2BP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 66kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Immunology, Signal Transduction
Swissprot #:
Q9Y6M1
User NOte:
Optimal dilutions for each application to be determined by the researcher.