SERPINA3 Antibody

ProSci
Product Code: PSI-18-819
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-819-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SERPINA3 antibody (18-819) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat liver using SERPINA3 antibody (18-819) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using SERPINA3 antibody (18-819) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using SERPINA3 antibody (18-819) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using SERPINA3 antibody (18-819) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat liver using SERPINA3 antibody (18-819) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SERPINA3 antibody (18-819) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SERPINA3 antibody (18-819) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Alpha-1-antichymotrypsin, ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3, Alpha-1-antichymotrypsin His-Pro-less, SERPINA3, AACT
Application Note:
WB: 1:200 - 1:1000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-423 of human SERPINA3 (NP_001076.2).
NCBI Gene ID #:
12
NCBI Official Name:
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
NCBI Official Symbol:
SERPINA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
P01011
User NOte:
Optimal dilutions for each application to be determined by the researcher.