ARF1 Antibody

ProSci
Product Code: PSI-18-828
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-828-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ARF1 antibody (18-828) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded mouse brain using ARF1 antibody (18-828) (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using ARF1 antibody (18-828) (40x lens).
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Immunohistochemistry of paraffin-embedded human prostate using ARF1 antibody (18-828) (40x lens).
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Immunofluorescence analysis of HeLa cells using ARF1 antibody (18-828) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ARF1 antibody (18-828) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse brain using ARF1 antibody (18-828) (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using ARF1 antibody (18-828) (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using ARF1 antibody (18-828) (40x lens).
Immunofluorescence analysis of HeLa cells using ARF1 antibody (18-828) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ADP-ribosylation factor 1, ARF1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human ARF1
NCBI Gene ID #:
375
NCBI Official Name:
ADP-ribosylation factor 1
NCBI Official Symbol:
ARF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P84077
User NOte:
Optimal dilutions for each application to be determined by the researcher.