MIP Antibody

ProSci
Product Code: PSI-18-849
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-849-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MIP antibody (18-849) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse brain using MIP antibody (18-849) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using MIP antibody (18-849) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using MIP antibody (18-849) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using MIP antibody (18-849) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using MIP antibody (18-849) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MIP antibody (18-849) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using MIP antibody (18-849) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
AQPCTRCT15, LIM126, MP26, MIP, lens fiber major intrinsic protein, aquaporin 0
Application Note:
WB: 1:500 - 1:1000

IHC: 1:100 - 1:200
Background:
Major intrinsic protein is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. The function of the fiber cell membrane protein encoded by this gene is undetermined, yet this protein is speculated to play a role in intracellular communication. The MIP protein is expressed in the ocular lens and is required for correct lens function. This gene has been mapped among aquaporins AQP2, AQP5, and AQP6, in a potential gene cluster at 12q13.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MIP (NP_036196.1).
NCBI Gene ID #:
4284
NCBI Official Name:
major intrinsic protein of lens fiber
NCBI Official Symbol:
MIP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P30301
User NOte:
Optimal dilutions for each application to be determined by the researcher.