DUT Antibody

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ProSci
Product Code: PSI-18-860
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-18-860-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DUT antibody (18-860) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
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Immunohistochemistry of paraffin-embedded rat spleen using DUT antibody (18-860) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using DUT antibody (18-860) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human appendix using DUT antibody (18-860) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U-2 OS cells using DUT Polyclonal Antibody (18-860) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using DUT antibody (18-860) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat spleen using DUT antibody (18-860) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using DUT antibody (18-860) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using DUT antibody (18-860) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using DUT Polyclonal Antibody (18-860) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
DUT, FLJ20622, dUTPase
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:200

IF: 1:100 - 1:200
Background:
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 89-252 of human DUT (NP_001020419.1).
NCBI Gene ID #:
1854
NCBI Official Name:
deoxyuridine triphosphatase
NCBI Official Symbol:
DUT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 24kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P33316
User NOte:
Optimal dilutions for each application to be determined by the researcher.