DNM1 Antibody

ProSci
Product Code: PSI-18-861
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-861-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using DNM1 antibody (18-861) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunofluorescence analysis of HeLa cells using DNM1 antibody (18-861).

Western blot analysis of extracts of various cell lines, using DNM1 antibody (18-861) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using DNM1 antibody (18-861).

Further Information

Additional Names:
DNM1, Dynamin, Dynamin-1, DNM, Dynamin 1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:100 - 1:200
Background:
This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human DNM1
NCBI Gene ID #:
1759
NCBI Official Name:
dynamin 1
NCBI Official Symbol:
DNM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 117kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Neuroscience, Signal Transduction
Swissprot #:
Q05193
User NOte:
Optimal dilutions for each application to be determined by the researcher.