CLDN3 Antibody

ProSci
Product Code: PSI-18-879
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-879-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CLDN3 antibody (18-879) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded mouse stomach using CLDN3 antibody (18-879) (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using CLDN3 antibody (18-879) (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using CLDN3 antibody (18-879) (40x lens).

Western blot analysis of extracts of various cell lines, using CLDN3 antibody (18-879) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse stomach using CLDN3 antibody (18-879) (40x lens).
Immunohistochemistry of paraffin-embedded human colon using CLDN3 antibody (18-879) (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CLDN3 antibody (18-879) (40x lens).

Further Information

Additional Names:
CLDN3, C7orf1, Claudin-3, CPE-R 2, Claudin 3, RVP1, CPE-R2, CPE-receptor 2, CPETR2, HRVP1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Background:
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human CLDN3
NCBI Gene ID #:
1365
NCBI Official Name:
claudin 3
NCBI Official Symbol:
CLDN3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 23kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
O15551
User NOte:
Optimal dilutions for each application to be determined by the researcher.