MMP10 Antibody

ProSci
Product Code: PSI-18-912
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-912-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MMP10 antibody (18-912) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat kidney using MMP10 antibody (18-912) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using MMP10 antibody (18-912) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH-3T3 cells using MMP10 Polyclonal Antibody (18-912) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using MMP10 antibody (18-912) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using MMP10 antibody (18-912) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MMP10 antibody (18-912) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using MMP10 Polyclonal Antibody (18-912) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
MMP10, SL-2, STMY2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down fibronectin, laminin, elastin, proteoglycan core protein, gelatins, and several types of collagen. The gene is part of a cluster of MMP genes on chromosome 11.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human MMP10
NCBI Gene ID #:
4319
NCBI Official Name:
matrix metallopeptidase 10 (stromelysin 2)
NCBI Official Symbol:
MMP10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 54kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle
Swissprot #:
P09238
User NOte:
Optimal dilutions for each application to be determined by the researcher.