MYL9 Antibody

ProSci
Product Code: PSI-18-915
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-915-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using MYL9 antibody (18-915) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunofluorescence analysis of Rat intestine using MYL9 antibody (18-915) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of Human colon carcinoma using MYL9 antibody (18-915) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of Mouse intestine using MYL9 antibody (18-915) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using MYL9 antibody (18-915) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of Rat intestine using MYL9 antibody (18-915) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Human colon carcinoma using MYL9 antibody (18-915) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Mouse intestine using MYL9 antibody (18-915) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
MYL9, 20 kDa myosin light chain, LC20, MLC-2C, MLC2, Myosin RLC, MRLC1, MYRL2
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MYL9 (NP_006088.2).
NCBI Gene ID #:
10398
NCBI Official Name:
myosin, light chain 9, regulatory
NCBI Official Symbol:
MYL9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P24844
User NOte:
Optimal dilutions for each application to be determined by the researcher.