N Cadherin Antibody, KO Validated

ProSci
Product Code: PSI-18-917
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-917-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using N Cadherin antibody (18-917) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 3
Western blot analysis of extracts from normal (control) and N Cadherin knockout (KO) HeLa cells, using N Cadherin antibody (18-917) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
3 / 3
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug N Cadherin antibody (18-917). Western blot was performed from the immunoprecipitate using N Cadherin antibody (18-917) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using N Cadherin antibody (18-917) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts from normal (control) and N Cadherin knockout (KO) HeLa cells, using N Cadherin antibody (18-917) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug N Cadherin antibody (18-917). Western blot was performed from the immunoprecipitate using N Cadherin antibody (18-917) at a dilition of 1:1000.

Further Information

Additional Names:
CDH2, CDw325, CDHN, Cadherin-2, CD325, CD325 antigen, N Cadherin, N-cadherin, NCAD, Neural cadherin, N-cadherin 1, Neural-cadherin
Application Note:
WB: 1:500 - 1:1000

IP: 1:50 - 1:100
Background:
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 750-850 of human N Cadherin (NP_001783.2).
NCBI Gene ID #:
1000
NCBI Official Name:
cadherin 2, type 1, N-cadherin (neuronal)
NCBI Official Symbol:
CDH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 135kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology, Signal Transduction, Stem Cell
Swissprot #:
P19022
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.