GRIN2B Antibody

ProSci
Product Code: PSI-18-922
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-922-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse brain, using GRIN2B antibody (18-922) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of rat brain using GRIN1 antibody (18-922) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of mouse brain using GRIN1 antibody (18-922) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse brain, using GRIN2B antibody (18-922) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of rat brain using GRIN1 antibody (18-922) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using GRIN1 antibody (18-922) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
GRIN2B, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, HGNC:4586, NMDAR2B, NR2B, hNR3, N-methyl-D-aspartate receptor subunit 2B
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1400 to the C-terminus of human GRIN2B (NP_000825.2).
NCBI Gene ID #:
2904
NCBI Official Name:
glutamate receptor, ionotropic, N-methyl D-aspartate 2B
NCBI Official Symbol:
GRIN2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 181kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
Q13224
User NOte:
Optimal dilutions for each application to be determined by the researcher.