PAWR Antibody

ProSci
Product Code: PSI-18-925
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-925-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PAWR antibody (18-925) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 3
Immunohistochemistry of paraffin-embedded rat pancreas using PAWR Antibody (18-925) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using PAWR Antibody (18-925) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using PAWR antibody (18-925) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat pancreas using PAWR Antibody (18-925) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PAWR Antibody (18-925) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
PAWR, PAR4, Par-4
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
The tumor suppressor WT1 represses and activates transcription. The protein encoded by this gene is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human PAWR
NCBI Gene ID #:
5074
NCBI Official Name:
PRKC, apoptosis, WT1, regulator
NCBI Official Symbol:
PAWR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 43kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle
Swissprot #:
Q96IZ0
User NOte:
Optimal dilutions for each application to be determined by the researcher.