PRDX3 Antibody

ProSci
Product Code: PSI-18-929
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-929-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PRDX3 antibody (18-929) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded rat kidney using PRDX3 antibody (18-929) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using PRDX3 antibody (18-929) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using PRDX3 antibody (18-929) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using PRDX3 antibody (18-929). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using PRDX3 antibody (18-929) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat kidney using PRDX3 antibody (18-929) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using PRDX3 antibody (18-929) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using PRDX3 antibody (18-929) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using PRDX3 antibody (18-929). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
PRDX3, AOP-1, AOP1, MER5, MGC104387, MGC24293, PRO1748, SP-22, HBC189, prx-III
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human PRDX3
NCBI Gene ID #:
10935
NCBI Official Name:
peroxiredoxin 3
NCBI Official Symbol:
PRDX3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25kDa
Purification:
Affinity purification
Research Area:
Cancer, Immunology, Neuroscience, Signal Transduction
Swissprot #:
P30048
User NOte:
Optimal dilutions for each application to be determined by the researcher.