TPM2 Antibody

ProSci
Product Code: PSI-18-940
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-940-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TPM2 antibody (18-940) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat testis using TPM2 antibody (18-940) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using TPM2 antibody (18-940) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human rectal cancer using TPM2 antibody (18-940) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using TPM2 antibody (18-940) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using TPM2 antibody (18-940) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TPM2 antibody (18-940) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using TPM2 antibody (18-940) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
TPM2, AMCD1, DA1, TMSB, DA2B, NEM4, HEL-S-273
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human TPM2
NCBI Gene ID #:
7169
NCBI Official Name:
tropomyosin 2 (beta)
NCBI Official Symbol:
TPM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P07951
User NOte:
Optimal dilutions for each application to be determined by the researcher.