Symmetric DiMethyl-Histone H3-R17 pAb

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ProSci
Product Code: PSI-18-964
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-964-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R17 antibody (18-964).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 4
Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R17 antibody (18-964).
3 / 4
Immunohistochemistry of paraffin-embedded human stomach using H3R17me2s antibody (18-964) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R17 antibody (18-964). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R17 antibody (18-964).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R17 antibody (18-964).
Immunohistochemistry of paraffin-embedded human stomach using H3R17me2s antibody (18-964) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R17 antibody (18-964). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
H3/b, H3FB, ERBB, ERBB1, HER1, H3/a, H3/c, H3/d, H3/f, H3/h
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic methylated peptide corresponding to residues surrounding Arg17 of human histone H3
Modifications:
Methylated
NCBI Gene ID #:
8356
NCBI Official Name:
histone cluster 1, H3d
NCBI Official Symbol:
H3R17me2s
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P68431
User NOte:
Optimal dilutions for each application to be determined by the researcher.