YKL-40 / CHI3L1 Antibody

ProSci
Product Code: PSI-18-974
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-974-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using YKL-40 / CHI3L1 antibody (18-974) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded mouse liver using YKL-40 / CHI3L1 antibody (18-974) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using YKL-40 / CHI3L1 antibody (18-974) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using YKL-40 / CHI3L1 antibody (18-974) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse liver using YKL-40 / CHI3L1 antibody (18-974) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using YKL-40 / CHI3L1 antibody (18-974) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
CHI3L1, DKFZp686N19119, FLJ38139, GP39, HC-gp39, HCGP-3P, YKL40, YYL-40, ASRT7, GP-39, CGP-39, YKL-40, hCGP-39
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-240 of human YKL-40 / CHI3L1 (NP_001267.2).
NCBI Gene ID #:
1116
NCBI Official Name:
chitinase 3-like 1 (cartilage glycoprotein-39)
NCBI Official Symbol:
CHI3L1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 43kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P36222
User NOte:
Optimal dilutions for each application to be determined by the researcher.